Sunday 18 September 2011

STORM supplement to support performance

Racing Blue STORM™ is a new patented feed supplement that supports muscle buffering function during training and racing. 

David Evans Science Pty Ltd offers a 10% discount on the online price of Racing Blue STORM™ for purchasers in Australian and New Zealand.

STORM™ combines ProCarnosine™ (sport patented beta alanine) with synergistic ingredients to enhance the absorption of ProCarnosine™ from the digestive tract and facilitate its uptake into muscle.   In essence, STORM™ provides the necessary building blocks for horses to synthesise carnosine within muscle that buffers lactic acid.  

Lactic acid builds up in muscle during exercise and contributes to fatigue, both during training and racing.  STORM™ helps horses to better tolerate lactic acid, which should allow them to maintain their maximum speed for longer.  This is relevant during racing, as it is often said that the horse that wins is the one that is last to slow down.  STORM™ also helps during training by bringing about an improved training response. 

STORM™ technology is backed by reputable scientific studies in horses and in depth research in human athletes with startling results.  Sport patented beta alanine is well established in the human sports sector as one of the most exciting breakthroughs in sports nutrition.  Launched in April 2011 in the UK, STORM™ is quickly proving itself within the equine racing sector.

If you would like more information or would like to discuss further, please visit the website www.racingblue.com or contact me by phone or email. 
davidevansscience@gmail.com

TREADMILL EXERCISE TESTS


EXERCISE TESTING ON THE TREADMILL
David Evans PhD BVSc (University of Sydney)
Honorary Associate Professor, Faculty of Veterinary Science, University of Sydney, NSW 2006, Australia
Director, Equine Health & Fitness Pty Ltd, www.equinehealthfitness.com.au, evans.david4@gmail.com


In this session the focus will be on use of heart rate and blood lactate measurements during and/or after treadmill exercise. More technically challenging measurements such as oxygen uptake can be used, but that measurement will probably remain in the domain of university research laboratories for some time, given its cost and technically demanding nature. The presentation will focus on the heart rate and blood lactate measurements that can be easily performed, and which will provide a meaningful assessment of the state of training (fitness) of a horse in a commercial setting.

Measuring fitness:
1.   Fitness for racing horses cannot be measured in horses standing quietly in their boxes
2.   Fitness tests require measurements during exercise tests
3.   Exercise tests can be conducted in treadmill laboratories, or on a racetrack
4.   The main measurements for fitness assessment are heart rate, blood lactate and oxygen uptake
5.   Heart rate measurements of fitness are the most suitable for field tests
6.   Fitness tests should help an owner and trainer by providing information about differences in fitness between horses, and changes in fitness in individual horses. Ideally a fitness test should also help guide the racing and training strategies for individual horses.
7.   Regular fitness tests (VHRmax) with heart rates in track gallops can assess performance, and evaluate poor performance. VHRmax can also help target appropriate training speeds to develop fitness in racehorses. Low values, or values that have decreased during training, indicate a need for a thorough veterinary examination for the possible causes.


The use of a treadmill for an exercise test can be complicated or simple. Simple methods are recommended, taking into account the rationale for the exercise test. What are the usual reasons for a treadmill exercise test?

1.     To enable upper airway endoscopy during exercise
2.     To measure the fitness of the horse

Fitness measurements are used to assess the responses to training and racing, and can help identify new clinical problems, and are used to assess horses with poor performance.

The protocols used for treadmill exercise testing vary greatly. It is not important that a protocol used in a university research setting is copied in a commercial setting. It is more important that a protocol is used that easily and quickly gives answers to the question being addressed.

Horses must be properly acclimated to the treadmill before conducting any measurements. This process should be patient, and only HR and lactate results in relaxed horses should be used for interpretation. Acclimation to trotting at 3.5-4.0 m/s (12-15 kph) and gallops at 6-8 m/s (20-26 kph) should be achieved over a 4-5 day period at least.

Unfortunately some horses enjoy treadmill exercise so much that their enthusiasm can make testing very difficult, as they push hard on the front restraint of the treadmill. Fortunately these cases are not common. As well, testing after a few days of rest should not be used, as horses are often excited, as they often are for racetrack exercise on Monday mornings.

There is no standard incline for testing. However, an incline of 10% (6 degrees) is often used. However, lower inclines can be used, and an incline of 2-3% has been suggested as most closely matching over-ground locomotion. However, it is not important to use a test that exactly equals over-ground locomotion, and it will never be fully achieved without jockey, saddle, bit in the mouth and so on. In any case, the recruitment of muscles during treadmill exercise is not exactly the same as in over-ground exercise. The treadmill does some of the work propelling the horse forward!

Most descriptions of treadmill exercise tests refer to standardised exercise tests that use a range of speeds. For example, a test could consist of trotting at 4 m/s for 5 minutes, followed by gallops for one minute at 6, 8 and 10 m/s. Heart rates could be measured during each step and during recovery. Heart rates during the test can then be used to calculate V140 or V200, the treadmill velocities at 140 or 200 beats per minute.

Ideally VHRmax should be measured in racehorse, but this necessitates measurement of maximum HR. Maximum HRs vary from 200-230 or more, and in trained racehorses treadmill speeds of 42 or more kph might be needed to record HRmax. An alternative is to record HRmax during a high speed track gallop.

A simple approach is to measure HRs during 5 minutes trotting, and record the lowest HR during that time. The lowest HR will most accurately represent the true HR because the effects of excitement are more likely to be eliminated. In racehorses, HRs might be from 90-140 bpm. HRs can also then be recorded during the last 10 seconds of one minute of gallop at 6 m/s. The result is HRs at trot and slow gallop HRs obtained in 6 minutes of exercise. Such a test would be appropriate for racehorses, eventers, jumpers, endurance horses, polo horses, and any horse that competes at a gallop. Dressage horses could probably be tested only at the trot.

Blood lactates could be collected via a jugular catheter during the exercise test, or collected 3-5 minutes after exercise by venipuncture, which is the author’s preference in a commercial setting.  Blood lactate will not accumulate in trained horses until treadmill speed exceeds 8-9 m/s, depending on the fitness of the horse. In one study, commercially trained thoroughbred horses had blood lactates ranging from 3-12 mmol/l in blood samples collected after two minutes treadmill gallop at 10 m/s. The best horses had the lowest blood lactate concentration (Evans et al., 1993).

These approaches to treadmill testing enable monitoring of fitness during training, and simple comparisons can be made between horses. The author has found that superior racehorses have lower HRs during trotting and slow gallops, and higher VHRmax during track gallops. Superior racehorses also have lower blood lactates after standardised treadmill exercise test that finishes with a gallop at 10 m/s. These observations are supported by results in several research reports in thoroughbred and standardbred horses.

Regular monitoring of HRs during simple treadmill tests can answer simple questions. Is the horse getting fitter? Has there been a sudden increase in HRs (indicating a possible subclinical problem)? Has the horse properly recovered from its race, and so is then ready for more training? Simple treadmill tests assist with answering these questions.

Identification of horses with poor physiological capacity for exercise and with poor racing performance can assist with management of the training and racing of those horses. Perhaps the training can be modified to promote greater fitness, which can be measured. Perhaps the horse can be sold, so reducing costs associated with maintenance of horses with lower likelihood of earning prize money. However, it is important that the tests are repeated several times, to ensure that the data are reliable. If results are not consistent, repeat the tests.


Reference

Evans, D.L., Harris, R.C. and Snow, D.H. (1993) Correlation of racing performance with blood lactate and heart rate in Thoroughbred horses. Equine Veterinary Journal, 25:441-445

Tyler, CM, Golland, LC, Evans, DL, Hodgson, DR and Rose, RJ (1996) Changes in maximum oxygen uptake during prolonged training, overtraining and detraining in horses. J Appl Physiol., 81:2244-2249.


PERFORMANCE-BASED SELECTION OF ATHLETIC HORSES


PERFORMANCE-BASED SELECTION OF ATHLETIC HORSES


One of the earliest themes in exercise physiology of horses was the selection of physiologically superior athletes. The concept of using a measurement that accurately predicted success in racing or other athletic events is still popular. In this session a review will be presented of the methods that have been used more recently. What is being measured? What is the evidence for those measurements?

Selection of horses based on a physiological measurement has usually focussed on cardiac measurements. Electrocardiographic assessment of heart size was popular for decades, and now cardiac ultrasound is used by some investigators. Muscle biospsies for assessment of fibre type distribution were briefly popular, but results are unreliable for racehorses.


There is good rationale for focus on the heart. Athletic performance depends on aerobic and anaerobic energy supply. The dependence on aerobic ATP resynthesis is higher in events with longer duration. It has been estimated that all thoroughbred races with durations exceeding 1000 m have greater dependence on aerobic than anaerobic energy supply. In a race with 2-3 minutes duration, aerobic energy might supply 70-80% of the total requirement. 

Assessment of anaerobic energy output in human athletes usually relies on 30 second bicycle or treadmill test of maximal energy output. No such equivalent test exists for horses, and more research is needed in this area.

Aerobic energy output is predicted by the Fick equation

Oxygen uptake = (HR x stoke volume) x peripheral oxygen use

Assessment of heart structure with cardiac ultrasound  in a resting horse as part of an examination to predict future performance attempts to predict stroke volume during exercise. This is a large extrapolation from resting measures of cardiac wall mass, end-diastolic volumes and indices of contractility. As well, indices of cardiac myocardial wall function during ultrasound depend on HR.  Other factors that could limit the accuracy of cardiac ultrasound to predict cardiac output at maximal exercise intensities include;

Absence of the effects of increase in blood volume due to splenic contraction
Cardiac contractility during high HRs
Effects of training is very different in individual horses
Measurements are not expressed relative to horse body weight or age – a serious limitation
Absence of maximum HR (varies by up to 15%), so excludes estimate of maximum cardiac output


Maximal heart rate can vary from 195 to 235 beats per minute, so in horses with the same sized heart, the blood blow from the heart during a race can vary by 20%. Assuming a stroke volume of 1.3 litres, the range of HRmax can explain a range of cardiac output of 50 litres per minute! This variation shows the difficulty of trying to predict future performance with cardiac ultrasound in yearlings to assess heart size. Of course the changes in the heart’s ability to pump blood is also likely to vary greatly between horses as they grow, train and exercise, due to differences in genetics, and changes in myocardial structure and function (contractility).

In human athletes, there is evidence to support a link between left ventricular mass and VO2max. It has also been suggested that VO2max correlates to athletic performance in horses. However, there was no a relationship between left ventricular size and VO2max in 6 Thoroughbreds exercising on a treadmill. However, this and most other studies focussed on flat race Thoroughbreds or Standardbreds that generally run over distances of less than 3,200 m (2 miles).
More recently a strong relationship between left ventricular mass and other measurements of cardiac size with VO2max was reported in a group of 18 Thoroughbred racehorses exercising on a high-speed treadmill (Young et al, 2002).  VO2max was significantly correlated with left ventricular (LV) internal diameter in diastole (r=0.71; P=0.001), estimated LV mass (r=0.78; P=0.0002) and LV short-axis area in diastole (r=0.69; P=0.003). When indices of heart size were indexed to body weight the correlation between VO2max and indices of heart size were lower or similar, (LV diastolic diameter (r=0.57; P=0.01), LV mass (r=0.78; P=0.0002) and LV short-axis area (r=0.69; P=0.003). 

Whilst a relationship between left ventricular dimensions and VO2max had been established, whether a similar relationship existed for heart size and athletic performance was still in doubt:  However, recent data from a large cross-sectional study of racehorses competing on the flat or over jumps in the United Kingdom did demonstrate a relationship between derived left ventricular mass and published rating (quality) in horses racing over longer distances in jump races (P£0.001), although the strength of the association with left ventricular mass was less for horses in flat races (Young et al..). Rather, left ventricular ejection fraction and left ventricular mass combined were positively associated with race rating in older flat race horses running over sprint (<1408m) and longer distances (>1408 m), explaining 25-35% of overall variation in performance, as well as being closely associated with performance in longer races over jumps (23%). Predicted differences between otherwise equivalent horses with small and large hearts was thus able to explain a significant proportion of the difference between elite and non-elite racehorse performance (Evans and Young, 2010). 

However, these results conflict with findings in 370 Thoroughbred yearlings, where there was no relationship between echocardiographic measurements of heart size and prospective race performance (Leadon et al., 1991). 

Use of echocardiography to predict future performance of racehorses should nevertheless still be used with caution, because the relative proportion of energy supply from aerobic metabolism probably varies widely. For example, in Thoroughbred races, with a range of 800-3200 metres distance, the relative contributions of aerobic energy output could be 40-80%.  With the possible exception of horses used for high level endurance riding, the technique is also likely to have limited value for horses other than those that race, as other skills are likely to be equally or more important influences on their athletic success than aerobic capacity. Additionally the level of skill required to obtain repeatable images of the equine left ventricle with an echocardiograph for this purpose is high and the confounding effects of gender, fitness, age and body size must always be taken into account. Prediction of maximal cardiac output and maximal oxygen uptake from estimates of stroke volume in a resting horse will be also confounded by variation in maximal heart rates during exercise.

Success of endurance horses will depend on superior aerobic fitness and economy of locomotion, reflected in lower HRs during exercise. 
Muscle biopsies have also been investigated for prediction of endurance horse performance (Rivero et al.)  . The disadvantage of muscle biopsies is their invasive nature, but a combination of muscle biopsy with assessment of heart rates during exercise should offer the best opportunity for accurate assessment of athletic potential in endurance horses.

What is the future for performance prediction if cardiac ultrasound measurements at rest are inaccurate. More studies are needed to investigate the use of heart rate and blood lactate measurements during field and treadmill exercise. Such studies will necessitate use of large numbers of commercially trained horses, with assessments scheduled during exercise undertaken before their first races. Treadmill or other mechanical testing equipment offers a methodology for such studies.

DNA analysis?
New commercial services offering genetic testing are now marketed. Each service should be scrutinised carefully for the evidence that supports the product, and thought given to the limitations of the service.


For example, a service that only estimates sprint capacity is probably ignoring a very important factor in the performance of most athletic horses; their aerobic capacity.
References
Evans D.L. and Young, L (2010) Cardiac responses to exercise and training. In: Cardiology of the Horse. Editor Marr, C. M., published by Elsevier Saunders, London
Young LE. Diseases of the heart and vessels. In: Hinchcliff KW, Kaneps AJ, Goer RJ, editors. Equine Sports Medicine and Surgery: Basic and Clinical Sciences of the Equine Athlete. Edinburgh: WB Saunders; 2003. p. 728–769.

Sampson SN, Tucker RL, Bayly WM. Relationship between VO2max, heart score and echocardiographic measurements obtained at rest and immediately following maximal exercise in thoroughbred horses. Equine VetJ Suppl 1999;30:190–194.

 Young LE, Marlin DJ, Deaton C, Brown-Feltner H, Roberts CA, Wood JLN. Heart size estimated by echocardiography correlates with maximal oxygen uptake. Equine Vet J Suppl
2002;34:467–472.

 Leadon D, McAllister H, Mullins E, Osborne M. Electrocardiographic and echocardiographic measurements and their relationships in Thoroughbred yearlings to subsequent performance. In: Persson SGB, Lindholm A, Jeffcott LB, editors. Equine Exercise Physiology 3. Davis, CA: ICCEP Publications; 1991. p. 18–22.

Training to avoid injury


Training to avoid injury
Many recent studies of adaptations to exercise and training have demonstrated that tendons, cartilage, bone and other musculoskeletal structures do adapt to the stimulus of regular exercise, or training. In young horses adaptations have been demonstrated, and these changes could help prevent injury during their racing careers in later years. Epidemiolgical studies have shown that racing success is dependent on the volume of exercise training, but there are also limits to the speeds and durations of exercise that can be sustained without resultant injury. It has also been demonstrated that the rate of increase in distances at high speeds is also an important risk factor for lameness, with higher rates resulting in higher risk. Exercise programs have also been used to prevent development of osteochondrosis. The overall conclusion is that exercise programs in young horses, coupled with low-risk training strategies in older horses, can reduce the risk of injury.